A one-pot enzymatic approach to the O-fluoroglucoside of N-methylanthranilate

作者：Caputi Lorenzo^*; Rejzek Martin; Louveau Thomas; O'Neill Ellis C; Hill Lionel; O**ourn Anne; Field Robert A

来源：Bioorganic & Medicinal Chemistry, 2013, 21(16): 4762-4767.

DOI：10.1016/j.bmc.2013.05.057

• Sugar nucleotide
• Biotransformation
• Fluorosugar
• Glucosyltransferase
• Natural product

In connection with prospective F-18-PET imaging studies, the potential for enzymatic synthesis of fluorine-labelled glycosides of small molecules was investigated. Approaches to the enzymatic synthesis of anomeric phosphates of D-gluco-configured fluorosugars proved ineffective. In contrast, starting in the D-galacto series and relying on the consecutive action of Escherichia colt galactokinase (GalK), galactose-1-phosphate uridylyltransferase (GalPUT), uridine-5'-diphosphogalactose 4-epimerase (GalE) and oat root glucosyltransferase (SAD10), a quick and effective synthesis of 6-deoxy-6-fluoro-D-glucosyl N-methylanthranilate ester was achieved.

• 出版日期2013-8-15