Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels

作者：Marin Victor; Kieffer Roland; Padmos Raymond; Aubin Tam Marie Eve

来源：Analytical Chemistry, 2016, 88(15): 7466-7470.

DOI：10.1021/acs.analchem.6b00926

We report a simple, cost-effective,, and reproducible method to form free-standing lipid bilayer membranes in micro devices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single a-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.

• 出版日期2016-8-2