Usefulness of haptoglobin and serum amyloid A proteins as biomarkers for atherothrombotic ischemic stroke diagnosis confirmation

作者：Brea David; Sobrino Tomas; Blanco Miguel; Fraga Maximo; Agulla Jesus; Rodriguez Yanez Manuel; Rodriguez Gonzalez Raquel; Perez de la Ossa Natalia; Leira Rogelio; Forteza Jeronimo; Davalos Antonio; Castillo Jose^*

来源：Atherosclerosis, 2009, 205(2): 561-567.

DOI：10.1016/j.atherosclerosis.2008.12.028

• Ischemic stroke classification
• Cardioembolic stroke
• Atherothrombotic stroke
• Proteomics

Objective: To identify protein biomarkers in order to classify ischemic stroke subtypes using proteomic analysis and immunoenzymatic tools for clinical validation.
Methods and results: We performed a proteomic analysis in serum samples of 24 patients with ischemic stroke (12 atherothrombotic patients and 12 cardioembolic patients). In this study, based on two-dimensional electrophoresis and mass spectrometry we found four spots whose expression intensity was at least four times stronger in atherothrombotic patients than in cardioembolic patients. These spots were identified as haptoglobin related protein, serum amyloid A (two spots) and haptoglobin alpha chain. We validated the possible value of haptoglobin and serum amyloid A in a larger series of patients (n = 262) with ischemic stroke using ELISA techniques. Haptoglobin levels > 1040 mu g/mL identified atherothrombotic patients with 95% sensitivity and 88% specificity whereas serum amyloid A levels > 160 mu g/mL identified atherothrombotic patients with 91% sensitivity and 83% specificity.
Conclusions: Haptoglobin and serum amyloid A are useful biomarkers for atherothrombotic ischemic stroke diagnosis confirmation.

• 出版日期2009-8