Acute Spill-Mimicking Exposure Effect of Hexavalent Chromium on the Pituitary-Ovarian Axis of a Teleost, Channa punctatus (Bloch)

作者：Mishra Ashish K; Mohanty B****ata^*

来源：Environmental Toxicology, 2014, 29(7): 733-739.

DOI：10.1002/tox.21799

• acute chromium exposure effect
• pituitary-ovarian axis
• histopathology
• immunocytochemistry
• 17 beta-estradiol assay
• Channa punctatus

Acute exposure to hexavalent chromium (as 10, 20, and 40 mg/L potassium dichromate for 96 h) adversely affected the pituitary-ovarian axis of a teleost Channa punctatus. The toxic impact of metal exposure on fish ovary was revealed in the form of increased percentage of atretic follicles, significantly in 20 mg/L and 40 mg/L exposure groups. The follicular atresia mostly occurred in vitellogenic (stage II and stage III) oocytes. Reduction of serum level of 17 beta-estradiol was also significant in 20 mg/L and 40 mg/L exposure groups. The increase of LH-immunointensity of pituitary gonadotrophs (LH beta-immunoreactive cells) and their hypertrophy was evident, significantly in fish of 40 mg/L exposed group. Thus, the present acute metal spill-mimicking laboratory study clearly demonstrated that short-term exposures to high doses of hexavalent chromium may disrupt reproduction of the fish and affect their population.

• 出版日期2014-5