Anti-avian influenza virus H9N2 activity of aqueous extracts of Zingiber officinalis (Ginger) and Allium sativum (Garlic) in chick embryos

作者：Rasool Amir; Khan Muti ur Rehman; Ali Muhammad Asad; Anjum Aftab Ahmad; Ahmed Ishtiaq; Aslam Asim; Mustafa Ghulam; Masood Saima; Ali Muhammad Amjad; Nawaz Muhammad

来源：Pakistan Journal of Pharmaceutical Sciences, 2017, 30(4): 1341-1344.

• Avian Influenza virus
• Zingiber officinalis
• Allium sativum
• antiviral
• cytotoxic
• chick embryo
• MTT assay

In the present study, anti-Avian influenza virus H9N2 activity of aqueous extracts (5, 10, 15, 20, 25%) of Zingiber officinalis and Allium sativum was evaluated. Embryo-toxicity was evaluated by histopathological scoring of Chorio-allantoic membrane of chick embryos. Cytotoxicity of extracts was determined by MTT assay on Vero cells. Aqueous extract of ginger had antiviral activity at 10, 15, 20 and 25% while garlic had activity at 15, 20 and 25%. Histopathological scoring of chorio-allantoic membrane for aqueous extracts (5, 10, 15, 20, 25%) of ginger (0.66 0.57, 1.33 0.57, 1.66 0.57, 2.66 0.57, 3.66 0.57, respectively) and garlic (1.00 0.00, 1.33 0.57, 2.00 0.00, 2.33 0.57, 3.66 0.57, respectively) was concentration dependant. MTT assay revealed cytotoxicity of both plants was also concentration dependent. Extracts of ginger (5, 10, 15, 20, 25%) had lower cytotoxicity (71, 59, 28, 22, 0 % cell survival, respectively) as compared to garlic (61, 36. 20, 11, 3% cell survival, respectively). Overall results revealed that concentration of aqueous extract of ginger (10%), showing antiviral activity against H9N2, was less toxic to vero cells (>50% cell survival). It is insinuated that ginger may have anti-Avian influenza virus H9N2 potential and its active compounds needs further investigations.

• 出版日期2017-7