Luminescent europium sensors for specific detection of 8-oxo-dGTP by time-gated fluorescence

作者：Fuchi Yasufumi; Fukuda Takashi; Sasaki Shigeki^*

来源：Bioorganic & Medicinal Chemistry, 2018, 26(12): 3254-3260.

DOI：10.1016/j.bmc.2018.04.052

• 8-oxo-dGTP
• Recognition molecule
• Europium complex
• Time-resolved fluorescence

The 9-hydroxy-1,3-diazaphenoxazine-2-one unit was conjugated with the Eu3+-cyclen complex through a linker. This diazaphenoxazine group was expected as an antenna unit for the excitation of europium ion, and a selective recognition site for 8-oxo-dGTP base. Among the synthesized three derivatives, the highest fluorescence emission was obtained by the complex constructed of an ethylene linker and the cyclen unit with three N, N-dimethylacetamide groups. The Eu3+-cyclen complex exhibited a selective response to the 8-oxo-dGTP in aqueous media by a time-resolved fluorescence assay.

• 出版日期2018-7-23