A New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices

作者：Valentin Vargas Alexis; Chorover Jon; Maier Raina M^*

来源：Journal of Microbiological Methods, 2013, 92(2): 173-177.

DOI：10.1016/j.mimet.2012.12.001

• DGGE
• Gel-to-gel variations
• Microbial ecology
• Community fingerprint
• Molecular standard

The Standard-Based Polynomial Interpolation (SBPIn) method is a new simple three-step protocol proposed to address common gel-to-gel variations for the comparison of sample profiles across multiple DGGE gels. The advantages of this method include no requirement for additional software or modification of the standard DGGE protocol.

• 出版日期2013-2-15