Potential for pH Monitoring the Enzyme-Catalyzed Hydrolysis of Esters with a Novel Nano-Engineered Fluorescent Probe

作者：Huan, Yanfu; Xun, Erna; Xue, Lina; Wei, Shigang; Wang, Jiaxin; Wang, Zhi^*

来源：Sensor Letters, 2012, 10(3-4): 948-+.

DOI：10.1166/sl.2012.2584

• Nanoengineered Fluorescent probe
• pH Probe
• Enzyme
• Hydrolysis

The pH sensitive squaraine dye was doped inside silica nanoparticles (SiNPs) using a reverse microemulsion method. After modifying the surface of the silica nanoparticles with a carboxyl group, the novel pH nanosensor was used to determinate the concentration of H+ in aqueous solution. The fluorescence intensity of the nanosensor decreased linearly as the pH decreased in the range of 5.72-3.01. Based on this phenomenon, a simple, rapid and specific method to quick determination of enzyme reaction kinetics was proposed and the influence factors associated with enzyme-catalyzed reaction have also been investigated. The modified nanosensor has been successfully applied as pH probe in monitoring the hydrolysis of 1, 1, 1-trifluoro-2-octanol acetate catalyzed by Aeropyrum pernix esterase (APE1547) and several other enzyme-catalyzed hydrolysis of esters. It's demonstrated that the novel nanosensor is sensitive to environmental pH and found to be a satisfactory pH probe that could have potential applications in monitoring the enzyme-catalyzed hydrolysis of esters.

• 出版日期2012-4
• 单位吉林大学