科研之友
微信
新浪微博
Facebook
分享链接
摘要
The intra and inter genetic polymorphisms and genetic relatedness of 12 generations selected 3 lines (HBW1, LBW, L); 7 generations selected 1 line (HBW2) and a control (C) line in Japanese quail were investigated by RAPD-PCR. Fourteen DNA samples (7 male-7 female) from each line were analyzed using 24 primers. One hundred ninety six polymorphic loci were amplified (150 to 2600 bp) and polymorphism rate was calculated as 99.49%. Genotypic polymorphism rates were calculated as 63.45%, 31.47%, 42.13%, 35.53% and 36.55% in C, LBW, HBW1, HBW2 and L, respectively. Average heterozygosis within lines (H(S)), total heterozygosis (H(T)) and average Shannon index (I) values were 0.169, 0.354 and 0.528, respectively. The genetic variation was highest in C and lowest in LBW line. The genetic identity within line varied from 0.747 (C) to 0.875 (DCA) and between lines varied from 0.603 (C-L) to 0.813 (HBW1-LBW). It was found that genetically similar selection line to base ancestor (C) is HBW2 line (0.667) while farthest is L line (0.494). Thus genetic variation within line and the genetic relationships among lines could be estimated successfully using RAPD-PCR in selected Japanese quail lines.
- 出版日期2010-9
