Involvement of protein kinase C signalling and mitogen-activated protein kinase in the amebocyte-producing organ of Biomphalaria glabrata (Mollusca)

作者：Salamat Zahra; Sullivan John T^*

来源：Developmental and Comparative Immunology, 2009, 33(6): 725-727.

DOI：10.1016/j.dci.2009.01.001

• Mitotic cell division
• Signal transduction
• Phorbol myristate acetate
• Snails
• Hemocytes
• Hematopoiesis

Mechanisms that regulate hemocyte production in molluscs, at either the organismal or cellular levels, are not well understood. In the present study, 24-h saline cultures of the amebocyte-producing organ (APO) of the schistosome-transmitting snail Biomphalaria glabrata were used to test for the potential involvement of protein kinase C (PKC) signalling in hematopoiesis. Exposure to phorbol myristate acetate (PMA), an activator of PKC, resulted in an increase in the number of dividing hematopoietic cells in APOs from schistosome-resistant Salvador snails. PMA-induced cell division was blocked by treatment with U0126, an inhibitor of the mitogen-activated protein kinase kinase, MEK1/2. These results suggest that PKC-induced activation of the mitogen-activated protein kinase, ERK1/2, is involved in cell division in the APO.

• 出版日期2009