Anti-Tumor Necrosis Factor Therapy Restores Peripheral Blood B-cell Subsets and CD40 Expression in Inflammatory Bowel Diseases

作者：Li Zhe; Vermeire Severine; Bullens Dominique; Ferrante Marc; Van Steen Kristel; Noman Maja; Bossuyt Xavier; Rutgeerts Paul; Ceuppens Jan L; Van Assche Gert^*

来源：Inflammatory Bowel Diseases, 2015, 21(12): 2787-2796.

DOI：10.1097/MIB.0000000000000554

• anti-TNF therapy
• B-cell subsets
• CD40
• inflammatory bowel diseases (IBD)

Background:Anti-tumor necrosis factor (TNF) therapy has become a standard therapy for severe inflammatory bowel diseases (IBD), but its effect on B lymphocytes is largely unexplored. In this study we investigated peripheral blood B cells, B-cell subsets, and CD40 expression in patients with IBD before and during anti-TNF therapy with infliximab (IFX).Methods:Blood was taken from healthy controls (n = 52) and patients with active IBD before (n = 46) and/or during anti-TNF therapy (n = 55). B-cell markers were detected by immunofluorescent staining and FACS analysis. Patients were classified as responders or nonresponders to anti-TNF therapy.Results:We found a numerical deficiency of circulating CD19(+) B cells, a lower activation state (CD40 expression) and lower proportions of CD5(+) B cells and IgM(+)IgD(+)CD27(+) preswitched memory cells among B cells in active patients with IBD before IFX therapy compared with healthy controls. IFX treatment increased CD19(+) B-cell numbers as well as the proportions of named B-cell subsets in responders but not in nonresponders. IFX more effectively upregulated CD40 expression in responders than in nonresponders. Restoration of B cells correlated with the biological response to therapy (C-reactive protein). Trough serum levels of IFX correlated with the number of B cells during therapy.Conclusions:A lower number of circulating B cells, a low CD40 expression, and a decrease in the proportion of CD5(+) and in the preswitched memory subset characterize active IBD. Restoration of these abnormalities correlates with the clinical response to anti-TNF therapy. The mechanism for this effect on B cells should be further explored.

• 出版日期2015-12