Anti-hypercalcemic effect of orally administered recombinant Saccharomyces cerevisiae expressing salmon calcitonin on hypercalcemic rats

作者：Sun Pingnan; Zhang Xuecheng^*; Zang Xiaonan; Zhou Xiaoling; Chen Yunsong; Arunakumara K K I U; Liang Bin

来源：Biotechnology Letters, 2007, 29(7): 1013-1018.

DOI：10.1007/s10529-007-9352-3

• hypercalcemia
• osteoclast
• Saccharomyces cerevisiae
• salmon calcitonin

Oral delivery of salmon calcitonin (sCT) to rats via a recombinant Saccharomyces cerevisiae was assessed. A synthetic sCT gene was cloned and expressed in S. cerevisiae yAGA2-sCT. Recombinant salmon calcitonin (rsCT) expression was detected by flow cytometry. The resorption activity of osteoclasts was inhibited by 3 x10(-6) M rsCT. Oral administration of 5 g lyophilized yAGA2-sCT/kg to hypercalcemic rats decreased serum calcium from 2.8 +/- 0.02-2.7 +/- 0.02 mM.

• 出版日期2007-7
• 单位中国海洋大学