Mitochondrial biogenesis in ventral spinal cord and nerve following sciatic nerve axotomy

作者：Huang, Wei; Wang, Tianbing; Jiang, Baoguo^*; Zheng, Ming^*

来源：International Journal of Clinical and Experimental Medicine, 2017, 10(10): 14386-14393.

• Mitochondrial biogenesis
• nerve
• motoneuron
• energy metabolism
• nerve regeneration

In the nerve system, mitochondria play pivotal roles in regulating various cellular processes and provide over 90% ATP supply to nerve activities. After nerve injury, cell bioenergetic status and energy metabolism related genes were significantly changed, but it is not clear whether mitochondrial biogenesis is involved in post-injury nerve degradation and regeneration processes. Here we show that mitochondrial biogenesis was down-regulated in spinal cord and up-regulated in proximal nerve after sciatic nerve injury, as indicated by the decreased or increased expressions of biogenesis biomarkers PGC-1 alpha, NRF-1, and TFAM. Mitochondrial to nuclear DNA ratio decreased in spinal cord and increased in proximal nerve. Moreover, mitochondrial morphology and ATP production were changed accordingly after nerve injury, with less, irregular mitochondria and reduced ATP content in spinal cord, and more, condensed mitochondria and elevated ATP content in proximal nerve. Our study revealed important but distinct changes of mitochondrial biogenesis in ventral spinal cord and nerve after peripheral nerve injury, and may provide treatment strategies for peripheral nerve injury through adjusting mitochondrial biogenesis and metabolic status.

• 出版日期2017
• 单位北京大学