An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer

作者：Chang KH; Lim JM^*; Kang SK; Lee BC; Moon SY; Hwang WS

来源：Fertility and Sterility, 2004, 82(4): 960-962.

DOI：10.1016/j.fertnstert.2004.03.052

Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca2+/Mg2+-free fusion medium and a serum-free medium being used for bovine embryos.

• 出版日期2004-10