Dependence of Cardiac Trabeculation on Neuregulin Signaling and Blood Flow in Zebrafish

作者：Peshkovsky Courtney; Totong Ronald; Yelon Deborah^*

来源：Developmental Dynamics, 2011, 240(2): 446-456.

DOI：10.1002/dvdy.22526

• zebrafish
• cardiac morphogenesis
• ventricular trabeculation
• myocardium
• endocardium
• cloche
• AG1478
• weak atrium

Maturation of the developing heart requires the structural elaboration of the embryonic ventricle through the process of trabeculation. Trabeculae form as the ventricular myocardium protrudes into the lumen of the chamber, thereby increasing muscle mass and altering functional output. Little is understood about the cellular basis for trabeculation and its genetic regulation. Here, we establish the utility of the zebrafish embryo for the analysis of the mechanisms driving trabeculation. In zebrafish, we can follow trabeculation in four dimensions and define morphologically discrete stages for the initiation, propagation, and network elaboration that form the ventricular trabeculae. We find that Neuregulin/ErbB signaling is required for the initial protrusion of the myocardium into the ventricular lumen. Additionally, we demonstrate that optimal blood flow through the ventricle is important for the advancement of trabeculation. Thus, our results indicate that the zebrafish provides a valuable model for investigating possible causes of congenital defects in trabeculation. Developmental Dynamics 240:446-456, 2011.

• 出版日期2011-2