Association of polymorphisms at the microRNA binding site of the caprine KITLG 3 '-UTR with litter size

作者：An, Xiaopeng; Song, Yuxuan; Bu, Shuhai; Ma, Haidong; Gao, Kexin; Hou, Jinxing; Wang, Shan; Lei, Zhang; Cao, Binyun^*

来源：Scientific Reports, 2016, 6(1): 25691.

DOI：10.1038/srep25691

• SINGLE-NUCLEOTIDE POLYMORPHISMS
• C-KIT
• TARGET RECOGNITION
• REGULATORY ROLE
• EXPRESSION
• LIGAND
• GENE
• FOLLICULOGENESIS
• PRINCIPLES
• RECEPTOR

This study identified three novel single nucleotide polymorphisms (SNPs) (c.1389C > T, c.1457A > C and c.1520G > A) in the caprine KITLG 3'-UTR through DNA sequencing. The three SNP loci were closely linked in Guanzhong dairy (GD) goats. Two alleles of the c.1457A > C SNP introduced two miRNA sites (chi-miR-204-5p and chi-miR-211). Individuals with combined genotype TT-CC-AA had a higher litter size compared with those with combined genotypes CC-AA-GG, TC-CC-GA and CC-AC-GG (P < 0.05). Luciferase assays showed that chi-miR-204-5p and chi-miR-211 suppressed luciferase expression in the presence of allele 1457A compared with negative control (NC) and allele 1457C (P < 0.05). Western blot revealed that KITLG significantly decreased in the granulosa cells (GCs) of genotype AA compared with that in the GCs of genotype CC and NC (P < 0.05). The KITLG mRNA levels of the CC-AA-GG carriers significantly decreased compared with those of the TT-CC-AA, TC-CC-GA and CC-AC-GG carriers. In addition, cell proliferation was reduced in haplotype C-A-G GCs compared with that in haplotype T-C-A GCs. These results suggest that SNPs c.1389C > T, c.1457A > C and c.1520G > A account for differences in the litter size of GD goats because chi-miR-204-5p and chi-miR-211 could change the expression levels of the KITLG gene and reduce GC proliferation.

• 出版日期2016-5-11
• 单位西北农林科技大学