Optimization of extraction of antioxidants from wheat bran (Triticum spp.) using response surface methodology

作者：Singh Barinderjit^*; Sharma Harish Kumar; Sarkar Bhavesh Chandra

来源：Journal of Food Science and Technology-Mysore, 2012, 49(3): 294-308.

DOI：10.1007/s13197-011-0276-5

• Wheat bran
• Free phenolic compound (FPC)
• Total flavonoid compound (TFC)
• Total antioxidant activity (TAA)
• Response surface methodology (RSM)
• Extraction

In this study, the optimal conditions for the extraction of antioxidants from hard winter wheat bran (WH711) were determined using response surface methodology (RSM). A central composite face centred design was used to investigate the effects of three independent variables, namely solvent composition (%v/v), extraction temperature (C) and time (minutes) on the responses; free phenolic content (FPC), total flavonoid content (TFC), total antioxidant activity (TAA), ferric reducing power (FRP), hydrogen peroxide scavenging activity (HPSA). Regression analysis showed that more than 90% of the variation was explained by the models of different responses. The optimal conditions for the antioxidantss extraction were found to be methanol concentration of 85%v/v, extraction temperature of 75 degrees C, extraction time of 45 min, for wheat bran. The experimental values of FPC, TFC, TAA, FRP and HPSA were 0.921 mg GAE/g bran (B), 0.4588 mg CE/g B, 0.01408 mM AAE/g B, 2.532 mM AAE/g B and 3.193 mM TE/g B, respectively which agreed with those predicted, thus indicating suitability of the model employed and the suitability of RSM in optimizing the extraction conditions.

• 出版日期2012-6