A DHHC-type zinc finger protein gene regulates shoot branching in Arabidopsis

作者：Xiang, Jing; Lin, Jianzhong; Tang, Dongying; Zhou, Bo; Guo, Ming; He, Reqing; Huang, Xingqun; Zhao, Xiaoying^*; Liu, Xuanming

来源：African Journal of Biotechnology, 2010, 9(45): 7759-7766.

• Arabidopsis
• DHHC-type zinc finger protein
• At5g04270
• shoot branching

Formation of plant architecture is a complicated biological phenomenon and is influenced by a variety of factors such as genotype, hormone, environment and nutrition. In this study, an activation-tagging mutant, scc10-D (suppressor of cry1cry2) grown in long-day (16-h light/8-h dark) condition showed enhanced shoot branching. The mRNA expression of six genes adjacent to the T-DNA insertion locus were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR), and the transcript level of a DHHC-type zinc finger protein gene, At5g04270, was found to increase markedly in the scc10-D mutant. The At5g04270 gene was then cloned and over-expressed in Arabidopsis. It was found that the At5g04270 over-expression lines had the features of enhanced shoot branching, while the T-DNA mutant of At5g04270 gene, SALK_006515, showed decreased shoot branching when compared to the wild type (WT). These results suggest that At5g04270 plays an important role in regulating shoot branching in Arabidopsis.

• 出版日期2010-11-8
• 单位长沙学院; 湖南大学