Deletion of Cyp6d4 does not alter toxicity of insecticides to Drosophila melanogaster

作者：Hardstone MC; Baker SA; Gao JW; Ewer J; Scott JG^*

来源：Pesticide Biochemistry and Physiology, 2006, 84(3): 236-242.

DOI：10.1016/j.pestbp.2005.07.008

• cytochrome P450 monooxygenases
• insecticide metabolism
• P element
• Crp6d4 null mutants
• insecta

Cytochrome P450-dependent monooxygenases are important in the activation and detoxilication of numerous insecticides. In this study, a Drosophila melanogaster Cyp6d4 null mutant was used to determine the role of this P450 in insecticide metabolism. This null mutant was generated by imprecise excision of a mobile P element located upstream to the P450 gene Cyp6d4. Comparative analysis between the non-functional mutant and relevant control strains shows that Cyp6d4 does not appear to be involved in the metabolism of chlorfenapyr, cypermethrin. diazinon, imidacloprid, malathion, oxamyl, parathion, or pyrethrum extract, even though these insecticides are known to be activated or detoxitied by P450-monooxygenases. No obvious

• 出版日期2006-3