Hic-5 affects proliferation, migration and invasion of B16 murine melanoma cells

作者：Noguchi Fumihito; Inui Shigeki^*; Nakajima Takeshi; Itami Satoshi

来源：Pigment Cell & Melanoma Research, 2012, 25(6): 773-782.

DOI：10.1111/pcmr.12005

• melanoma
• Hic5 protein
• mouse
• RhoA GTP-binding protein
• neoplasm metastasis

Hic-5 is a shuttling protein between the cell membrane and the nucleus which functions as a focal adhesion adaptor protein and a nuclear receptor coactivator. Although several studies have shown its involvement in other types of cancer, the role of Hic-5 in melanoma is unknown. Herein, we show for the first time that Hic-5 is expressed in B16-F1 murine melanoma cells. To determine its function in melanoma cells, we used shRNA-mediated RNA interference and established stable clones with down-regulated Hic-5 expression. These clones had impaired growth and metastatic potential compared with controls in vivo, which correlated with decreased proliferation, migration and invasion in vitro. Moreover, silencing of Hic-5 expression in B16-F1 activated RhoA with an amoeboid phenotypic change, indicating that Hic-5 is a key regulator of B16-F1 metastasis in the context of Rho-dependent motility. These results provide new evidence that Hic-5 is a possible molecular target for treatment of melanoma.

• 出版日期2012-11