Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors

作者：Park Eun Ji; Lee Kyung Soo; Lee Kang Choon; Na Dong Hee^*

来源：Electrophoresis, 2010, 31(22): 3771-3774.

DOI：10.1002/elps.201000302

• Granulocyte-colony stimulating factor
• Microchip CGE
• PEG-modification of protein

The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.

• 出版日期2010-11