A novel multiplex assay amplifying 13 Y-STRs characterized by rapid and moderate mutation rate

作者：Rogalla Urszula^*; Wozniak Marcin; Swobodzinski Jacek; Derenko Miroslava; Malyarchuk Boris A; Dambueva Irina; Kozinski Marek; Kubica Jacek; Grzybowski Tomasz

来源：Forensic Science International-Genetics, 2015, 15: 49-55.

DOI：10.1016/j.fsigen.2014.11.004

• RM Y-STRs
• Y chromosome
• Individual identification
• Buryats

As microsatellites located on Y chromosome mutate with different rates, they may be exploited in evolutionary studies, genealogical testing of a variety of populations and even, as proven recently, aid individual identification. Currently available commercial Y-STR kits encompass mostly low to moderately mutating loci, making them a perfect choice for the first two applications. Some attempts have been made so far to utilize Y-STRs to provide a discriminatory tool for forensic purposes. Although all 13 rapidly mutating Y-STRs were already multiplexed, no single assay based on single-copy markers allowing at least a portion of close male relatives to be differentiated from one another is available. To fill in the blanks, we constructed and validated an assay comprised of single-copy Y-STR markers only with a mutation rate ranging from 8 x 10 x(-3) to 1 x 10(-2). Performance of the resulting combination of nine RM Y-STRs and four moderately mutating ones was tested on 361 father-son pairs and 1326 males from 9 populations revealing an overall mutation rate of 1.607 x 10(-1) for the assay as a whole. Application of the proposed 13 Y-STR set to differentiation of haplotypes present among homogenous population of Buryats resulted in a threefold increase of discrimination as compared with 10 Y-STRs from the PowerPlex (R) Y.

• 出版日期2015-3