A new gene Bph33(t) conferring resistance to brown planthopper (BPH), Nilaparvata lugens (Stal) in rice line RP2068-18-3-5

作者：Naik Sabhavat Bhaskar; Divya Dhanasekar; Sahu Nihar; Sundaram Raman Meenakshi; Sarao Preetinder Singh; Singh Kuldeep; Lakshmi Vattikuti Jhansi; Bentur Jagadish Sanmallappa^*

来源：Euphytica, 2018, 214(3): UNSP 53.

DOI：10.1007/s10681-018-2131-5

• Brown planthopper (BPH)
• Resistance gene
• Bph33(t)
• SSR marker
• Heat shock protein

The rice brown planthopper (BPH) Nilaparvata lugens (Stal) is one of the major pests of rice across Asia. Host-plant resistance is the most ecologically acceptable means to manage this pest. A rice breeding line RP2068-18-3-5 (RP2068) derived from the land race Velluthacheera is reported to be resistant to BPH populations across India. We identified a new R gene [Bph33(t)] in this line using advanced generation RILs derived from TN1 x RP2068 cross through phenotyping at two locations and linkage analysis with 99 polymorphic SSR markers. QTL analysis through IciMapping identified at least two major QTL on chromosome 1 influencing seedling damage score in seed box screening, honey dew excretion by adults and nymphal survival. Since no BPH R gene has been reported on chromosome 1, we designate this locus as a new gene Bph33(t) which accounted for over 20% of phenotypic variance. Scanning the region for candidate gene suggested two likely candidates a leucine rich repeat (LRR) gene and a heat shock protein (HSP) coding gene. Expression profiling of the two genes in the two contrasting parents and RILs showed induction of the HSP gene (LOC_Os01g42190.1) at 6 h after infestation while LRR gene did not show such induction. It is likely that the HSP represented Bph33(t).

• 出版日期2018-3