AFM imaging reveals the tetrameric structure of the TRPM8 channel

作者：Stewart Andrew P; Egressy Kinga; Lim Annabel; Edwardson J Michael^*

来源：Biochemical and Biophysical Research Communications, 2010, 394(2): 383-386.

DOI：10.1016/j.bbrc.2010.03.027

• Transient receptor potential channels
• TRPM8
• Channel assembly
• Subunit stoichiometry
• Atomic force microscopy

Several members of the transient receptor potential (TRP) channel superfamily have been shown to assemble as tetramers. Here we have determined the subunit stoichiometry of the transient receptor potential M8 (TRPM8) channel using atomic force microscopy (AFM). TRPM8 channels were isolated from transfected cells, and complexes were formed between the channels and antibodies against a V5 epitope tag present on each subunit. The complexes were then subjected to AFM imaging. A frequency distribution of the molecular volumes of antibody decorated channels had a peak at 1305 nm(3), close to the expected size of a TRPM8 tetramer. The frequency distribution of angles between pairs of bound antibodies had two peaks, at 93 degrees and 172 degrees, confirming that the channel assembles as a tetramer. We suggest that this assembly pattern is common to all members of the TRP channel superfamily.

• 出版日期2010-4-2