A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells

作者：Mei Yuping; Wang Yuyan; Kumari Priti; Shetty Amol Carl; Clark David; Gable Tyler; MacKerell Alexander D; Ma Mark Z; Weber David J; Yang Austin J; Edelman Martin J; Mao Li^*

来源：Nature Communications, 2015, 6(1): 7316.

DOI：10.1038/ncomms8316

PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

• 出版日期2015-6
• 单位北京大学