Anticancer activity of polysaccharide from Glehnia littoralis on human lung cancer cell line A549

作者：Wu, Jun^*; Gao, Weiping; Song, Zhuoyue; Xiong, Qingping; Xu, Yingtao; Han, Yun; Yuan, Jun; Zhang, Rong; Cheng, Yunbo; Fang, Jiansong; Li, Weirong; Wang, Qi^*

来源：International Journal of Biological Macromolecules, 2018, 106: 464-472.

DOI：10.1016/j.ijbiomac.2017.08.033

• Polysaccharide
• Glehnia littoralis
• Lung cancer

The purpose of this study was to investigate the anticancer activity of polysaccharide (PGL) from Glehnia littoralis on human lung cancer cell line A549. Based on Mff assay, the results suggested that PGL could significantly reduce A549 cells proliferation in a time- and dose-dependent manner. In addition, PGL displayed an inhibitory activity for the A549 cells migration in Transwell migration assay. The results from both flow cytometry analysis and Hochst 3342 staining of apoptotic cells indicated that PGL could promote apoptosis, and induce cycle arrest of A549 cells. Moreover, immunofluorescence assay elucidated PGL could also down-regulate expression of proliferating cell nuclear antigen (PCNA). Overall, these results showed that PGL exerts a strong anticancer action through inhibiting the A549 cells migration, proliferation and inducing cell apoptosis. It could be a new source of natural anticancer agent against lung cancer with potential value in supplements and medicine.

• 出版日期2018-1
• 单位滨州医学院; 淮阴工学院; 山东中医药大学; 广州中医药大学; 徐州医科大学