Aging-Dependent Regulation of Antioxidant Enzymes and Redox Status in Chronically Loaded Rat Dorsiflexor Muscles

作者：Ryan Michael J; Dudash Holly J; Docherty Megan; Geronilla Kenneth B; Baker Brent A; Haff G Gregory; Cutlip Robert G; Always Stephen E^*

来源：Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 2008, 63(10): 1015-1026.

DOI：10.1093/gerona/63.10.1015

• Exercise
• Oxidative stress
• Sarcopenia
• Superoxide dismutase

This study compares changes in the pro-oxidant production and buffering capacity in young and aged skeletal muscle after exposure to chronic repetitive loading (RL). The dorsiflexors from one limb of young and aged rats were loaded 3 times/week for 4.5 weeks using 80 maximal stretch-shortening contractions per session. RL increased H2O2 in tibialis anterior muscles of young and aged rats and decreased the ratio of reduced/oxidized glutathione and lipid peroxidation in aged but not young adult animals. Glutathione peroxidase (GPx) activity decreased whereas catalase activity increased with RL in muscles from young and aged rats. RL increased CuZn superoxide disumutase (SOD) and Mn SOD protein concentration and CuZn SOD activity in muscles from young but not aged animals. There were no changes in protein content for GPx-1 and catalase or messenger RNA for any of the enzymes studied. These data show that aging reduces the adaptive capacity of muscles to buffer increased pro-oxidants imposed by chronic RL.

• 出版日期2008-10