Molecular defense response of mycorrhizal bean plants infected with Rhizoctonia solani

作者：Hafez Elsayed E; Abdel Fattah Gamal M; El Haddad Safwat A; Rashad Younes M^*

来源：Annals of Microbiology, 2013, 63(3): 1195-1203.

DOI：10.1007/s13213-012-0578-5

• Arbuscular mycorrhizal fungi
• beta-1,3-Endoglucanase
• Chitinase
• Differential display technique
• Gene expression and root rot

A time course study was conducted to investigate disease development and molecular defense response in common bean (Phaseolus vulgaris L.) plants colonized by a mixture of five arbuscular mycorrhizal (AM) fungi, namely, Glomus mosseae, G. intraradices, G. clarum, Gigaspora gigantea, and Gigaspora margarita, and post-infected with the soil-borne pathogen Rhizoctonia solani. Results showed that pre-colonization of bean plants by AM fungi significantly reduced disease severity and disease incidence. DNA fingerprinting using the differential display technique revealed a genetic polymorphism (86.8 %) in bean plants that resulted from the colonization by AM fungi. Two genetic mechanisms were recorded: (1) switching on of new genes and (2) induction of other active genes, including the defense genes chitinase and beta-1,3-glucanase, to a highly expressed state.

• 出版日期2013-9
• 单位南宁师范大学