Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity

作者：Chen Yan; Leng Jie; Wang Yongqi; Xiao Weiming; Xi Juqun; Ding Yanbing; Qian Li; Pan Xingyuan; Ji Mingchun; Gong Weijuan^*

来源：Biochemical and Biophysical Research Communications, 2015, 463(3): 336-343.

DOI：10.1016/j.bbrc.2015.05.065

• Chitosan nanoparticle
• NKG2D
• IL-15
• Gene vaccine
• Tumor

Nanoparticles are becoming promising carriers for gene delivery because of their high capacity in gene loading and low cell cytotoxicity. In this study, a chitosan-based nanoparticle encapsulated within a recombinant pcDNA3.1-dsNKG2D-IL-15 plasmid was generated. The fused dsNKG2D-IL-15 gene fragment consisted of double extracellular domains of NKG2D with IL-15 gene at downstream. The average diameter of the gene nanoparticles ranged from 200 nm to 400 nm, with mean zeta potential value of 53.8 +/- 6.56 mV. The nanoparticles which were loaded with the dsNKG2D-IL-15 gene were uptaken by tumor cells with low cytotoxicity. Tumor cells pre-transfected by gene nanopartilces stimulated NK and T cells in vitro. Intramuscular injection of gene nanoparticles suppressed tumor growth and prolonged survival of tumor-bearing mice through activation of NK and CD8(+) T cells. Thus, chitosan-based nanoparticle delivery of dsNKG2D-IL-15 gene vaccine can be potentially used for tumor therapy.

• 出版日期2015-7-31
• 单位扬州大学