An In Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements

作者：Rodriguez Ismael A^*; Rozas Ferrara Carlos A; Campos Sanchez Fernando; Alaminos Miguel; Uribe Echevarria Jorge; Campos Antonio

来源：Journal of Adhesive Dentistry, 2013, 15(6): 541-546.

DOI：10.3290/j.jad.a29588

• glass-ionomer cements
• biocompatibility
• intracellular
• electron probe microanalysis
• human gingival fibroblast
• LDH

Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). %26lt;br%26gt;Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton x (positive control) were used. %26lt;br%26gt;Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RM-GIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. %26lt;br%26gt;Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts.

• 出版日期2013