Altered rPrP substrate structures and their influence on real-time quaking induced conversion reactions

作者：Cheng Keding; Sloan Angela; Waitt Brooks; Vendramelli Robert; Godal Debra; Simon Sharon L R; O'Neil Joe; Carpenter Michael; Jackson Dave; Eastlake Jane; Mallinson Gary; Knox J David^*

来源：Protein Expression and Purification, 2018, 143: 20-27.

DOI：10.1016/j.pep.2017.10.007

• Prion detection
• Recombinant prion protein (rPrP)
• RT-QuIC
• Circular dichroism (CD) spectropolarimetry
• HPLC
• FPLC

Background: Bacterially-produced recombinant prion protein (rPrP) has traditionally been used for in vitro fibrillation assays and reagent development for prion disease research. In recent years, it has also been used as a substrate for real-time quaking-induced conversion (RT-QuIC), a very sensitive method of detecting the presence of the misfolded, disease-associated isoform of the prion protein (PrPd). Multi-centre trials have demonstrated that RT-QuIC is a suitably reliable and robust technique for clinical practice; however, in the absence of a commercial supplier of rPrP as a substrate for RT-QuIC, laboratories have been required to independently generate this key component of the assay. No harmonized method for producing the protein has been agreed upon, in part due to the variety of substrates that have been applied in RT-QuIC.
Methods: This study examines the effects of two different rPrP refolding protocols on the production, QuIC performance, and structure characteristics of two constructs of rPrP commonly used in QuIC: full length hamster and a sheep-hamster chimeric rPrP.
Results: Under the described conditions, the best performing substrate was the chimeric sheep-hamster rPrP produced by shorter guanidine-HCI exposure and faster gradient elution.
Conclusions: The observation that different rPrP production protocols influence QuIC performance indicates that caution should be exercised when comparing inter-laboratory QuIC results.

• 出版日期2018-3