Membrane attack complex generation increases as a function of time in stored blood

作者：Hu X; Patel R P; Weinberg J A; Marques M B; Ramos T N; Barnum S R^*

来源：Transfusion Medicine, 2014, 24(2): 114-116.

DOI：10.1111/tme.12109

• complement
• extrinsic protease pathway
• membrane attack complex

ObjectiveTo determine if the complement system, a potent mediator of inflammation, contributes to haemolysis during red blood cell (RBC) storage.
BackgroundRBCs in storage undergo structural and biochemical changes that may result in adverse patient outcomes post-transfusion. Complement activation on leukodepletion and during storage may contribute to the RBC storage lesion.
Methods/MaterialsWe performed a cross-sectional analysis of aliquots of leukoreduced RBC units, stored for 1-6weeks, for the levels of C3a, C5a, Bb, iC3b, C4d and C5b-9 [membrane attack complex (MAC)] by enzyme-linked immunosorbent assay (ELISA).
ResultsWe observed that only MAC levels significantly increased in RBC units as a function of storage time. We also observed that the level of C5b-9 bound to RBCs increased as a function of storage time.
ConclusionMAC levels increased over time, suggesting that MAC is the primary complement-mediated contributor to changes in stored RBCs. Inhibition of the terminal complement pathway may stabilise RBC functionality and extend shelf life.

• 出版日期2014-4