Alkaline phosphatase labeled antibody-based electrochemical biosensor for sensitive HE4 tumor marker detection

作者：Cadkova Michaela; Dvorakova Veronika; Metelka Radovan; Bilkova Zuzana; Korecka Lucie^*

来源：Electrochemistry Communications, 2015, 59: 1-4.

DOI：10.1016/j.elecom.2015.06.014

• Electrochemical immunosensor
• Magnetic particles
• Tumor biomarker HE4
• Alkaline phosphatase

Quantitative determination of the serum level of human epididymis protein 4 (HE4), a tumor marker for ovarian carcinoma, has come to the fore of interest mainly due to the possibility for its detection in early stages of the disease when the sensing of other biomarkers is limited. We present a simple ELISA based approach for rapid HE4 detection including its immunomagnetic capturing accompanied by sensitive electrochemical detection of the electroactive product formed after enzymatic conversion of the substrate by alkaline phosphatase used as a label of anti-HE4 IgG. The proposed immunosensor offers stability in time and due to excellent limit of detection at 6.8 fM HE4, and limit of quantification of 23 fM meets the requirements for the early detection of this biomarker.

• 出版日期2015-10