Emergence and Transmission of Arbovirus Evolutionary Intermediates with Epidemic Potential

作者：Stapleford Kenneth A; Coffey Lark L; Lay Sreyrath; Borderia Antonio V; Duong Veasna; Isakov Ofer; Rozen Gagnon Kathryn; Arias Goeta Camilo; Blanc Herve; Beaucourt Stephanie; Haliloglu Turkan; Schmitt Christine; Bonne Isabelle; Ben Tal Nir; Shomron Noam; Failloux Anna Bella; Buchy Philippe; Vignuzzi Marco^*

来源：Cell Host & Microbe, 2014, 15(6): 706-716.

DOI：10.1016/j.chom.2014.05.008

The high replication and mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict and prevent epidemics. By studying the spatial and temporal evolution of chikungunya virus during natural transmission between mosquitoes and mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut and salivary gland pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapitulated the emergence of a recent epidemic strain of chikungunya and identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito and mammalian hosts by altering virion stability and fusogenic activity. Thus, virus evolutionary trajectories can be predicted and studied in the short term before new variants displace currently circulating strains.

• 出版日期2014-6-11