Degraded Iota-Carrageenan Can Induce Apoptosis in Human Osteosarcoma Cells Via the Wnt/-Catenin Signaling Pathway

作者：Jin Zhe^*; Han Ya Xin; Han Xiao Rui

来源：Nutrition and Cancer-An International Journal, 2013, 65(1): 126-131.

DOI：10.1080/01635581.2013.741753

Osteosarcoma (OS) is a high-grade malignant bone tumor. Therefore, using both in vitro and in vivo assays, the effects of degraded iota-Carrageenan (-CGN) on a human osteosarcoma cell line, HOS, were examined. Degraded -CGN was observed to induce apoptosis and G1 phase arrest in HOS cells. Moreover, degraded -CGN suppressed tumor growth in established xenograft tumor models. Accordingly, the survival rate of these mice was significantly higher than that of mice bearing tumors treated with native -CGN or PBS. In addition, the formation of intratumoral microvessels was inhibited following treatment with degraded -CGN. In Western blot assays, degraded -CGN was found to inhibit the Wnt/-catenin signaling pathway. Overall, these studies demonstrate the antitumor activity of degraded -CGN toward the OS cell line, HOS. Moreover, valuable insight into the mechanisms mediated by degraded -CGN was obtained, potentially leading to the identification of novel treatments for OS. However, additional studies are needed to confirm these results in other cell types, particularly in human umbilical vein endothelial cells.

• 出版日期2013-1-1
• 单位中国医科大学