Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror

作者:Piyawattanametha W; Barretto RPJ; Ko TH; Flu**erg BA; Cocker ED; Ra HJ; Lee DS; Solgaard O; Schnitzer MJ*
来源:Optics Letters, 2006, 31(13): 2018-2020.
DOI:10.1364/OL.31.002018

摘要

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm X 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of similar to 16 degrees. Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.

  • 出版日期2006-7-1