Mitochondria-Targeted DNA Nanoprobe for Real-Time Imaging and Simultaneous Quantification of Ca2+ and pH in Neurons

作者：Liu, Zhichao; Pei, Hao; Zhang, Limin; Tian, Yang^*

来源：ACS Nano, 2018, 12(12): 12357-12368.

DOI：10.1021/acsnano.8b06322

• DNA nanoprobe
• mitochondria
• calcium
• pH
• neuron imaging
• real-time

Herein, a single highly selective DNA nanoprobe was designed and created for the real-time imaging and simultaneous quantification of two kinds of biological species using Ca2+ and pH; the molecules were selected as models because of their close relationship with cellular functions and diseases. A Ca2+ fluorescent probe was synthesized and assembled onto a DNA nanostructure together with pH-responsive, inner-reference, and mitochondria-targeted molecules. This nanoprobe with high spatial resolution, together with long-term fluorescent and structural stability, powerfully tracked pH and Ca2+ dynamics at the same localization in mitochondria in response to O-2(center dot-)-induced oxidative stress and aggregated amyloid beta (A beta) stimulation with a temporal resolution of milliseconds. Using this tool, we discovered that O-2(center dot-) and A beta triggered transitory cytoplasmic acidosis and then activated acid-sensing ion channel la (ASIC1a) in the mitochondrial membrane, leading to mitochondrial Ca2+ overload and pH abnormalities, which contribute to neuron death. Moreover, psalmotoxin 1 effectively protected against O-2(center dot-) - and A beta-induced neuron injury.

• 出版日期2018-12
• 单位华东师范大学