Asiatic Acid Reduces Blood Pressure by Enhancing Nitric Oxide Bioavailability with Modulation of eNOS and p47(phox) Expression in L-NAME-induced Hypertensive Rats

作者：Bunbupha Sarawoot; Pakdeechote Poungrat^*; Kukongviriyapan Upa; Prachaney Parichat; Kukongviriyapan Veerapol

来源：Phytotherapy Research, 2014, 28(10): 1506-1512.

DOI：10.1002/ptr.5156

• hypertension
• nitric oxide
• asiatic acid
• oxidative stress
• vascular dysfunction

We investigated the effect of asiatic acid (AA) on hemodynamic status, vascular function, oxidative stress markers, endothelial nitric oxide synthase (eNOS), and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit expression in N-nitro-l-arginine methyl ester hydrochloride (l-NAME)-induced hypertensive rats. Male Sprague-Dawley rats treated with l-NAME (40mg/kg/day) in drinking water for 5weeks showed significant increases in mean arterial pressure, heart rate, hindlimb vascular resistance, vascular dysfunction, superoxide anion (O-2(center dot-)) production, and plasma malondialdehyde. Moreover, NO metabolite (NOx) levels were reduced, aortic eNOS expression was downregulated, and NADPH oxidase subunit p47(phox) was upregulated in hypertensive rats (p<0.05). Hypertensive rats that were administered AA (10 or 20mg/kg/day) for the last 2weeks of the study showed significant improvement in hemodynamic status and vascular function. The antihypertensive effects of AA were associated with elevated plasma NOx levels, together with upregulation of eNOS expression. Decreased vascular O-2(center dot-) production, consistent with downregulation of p47(phox) expression, was also observed after AA treatment. Our results are therefore consistent with a model whereby AA reduces blood pressure by enhancing NO bioavailability.

• 出版日期2014-10