Marker-free dual-axis tilt series alignment

作者：Winkler Hanspeter^*; Taylor Kenneth A

来源：Journal of Structural Biology, 2013, 182(2): 117-124.

DOI：10.1016/j.jsb.2013.02.004

• Electron tomography
• Image registration
• Tilt series alignment
• Dual-axis tilt series

Dual-axis tilt series in electron tomography are collected by successively tilting the object about two approximately orthogonal tilt axes. Here we report on the extension of marker-free image registration based on cross-correlation techniques to dual-axis tilt series. A simultaneous geometry refinement yields accurate parameters for the computation of the final reconstruction. Both, image registration and 3D-reconstruction operate on the combined data from the paired single axis series rather than computing individual single axis tomograms followed by a separate combination step. We show that with simultaneous registration and reconstruction of dual-axis tilt series, tomograms with higher resolution are obtained than by merging separately computed tomograms.

• 出版日期2013-5