Apiaceous vegetable intake modulates expression of DNA damage response genes and microRNA in the rat colon

作者：Kim Jae Kyeom; Gallaher Daniel D; Trudo Sabrina P^*

来源：Journal of Functional Foods, 2018, 45: 138-145.

DOI：10.1016/j.jff.2018.04.003

• 2-Amino-1-methyl-6-phenylimidazo[4,5-b]
• pyridine
• Apiaceous vegetables
• Ccndl
• Celery
• microRNA
• Parsnip

We examined chemo-preventive mechanisms of apiaceous vegetable intake against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Rats fed either a purified diet, or purified diet supplemented with 21% apiaceous vegetables (API), were injected with PhIP after 6 d of feeding and colons harvested 24 h later. There was a trend for increased colonic mRNA expression of P-glycoprotein, a PhIP efflux transporter. No DNA damage response genes were up-regulated by API but 13 of these genes were down-regulated, compared to the positive control group, which may have been due to formation of fewer PhIP-DNA adducts. In colonic miRNA profiling, 11 out of 421 miRNAs were modulated in the API group, 3 of which target 19 mRNA5 involved in cancer signaling pathways. API increased miR-19a, which targets Ccndl, a potential oncogene responsible for G1-S cell cycle progression. In conclusion, apiaceous vegetable intake may be chemopreventive through modification of miRNA expression as well as P-glycoprotein expression in colon.

• 出版日期2018-6