Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

作者：Nicodemo Mariana de Castro; das Nevesii Lia Reno; Aguiar Josafa Carvalho; Brito Flaviane de Souza; Ferreira Isabelle; Sant'Anna Luciana Barros; Raniero Leandro Jose; Lopes Martins Rodrigo Alvaro; Barja Paulo Roxo; Lo Schiavo Arisawa Emilia Angela

来源：Acta Cirurgica Brasileira, 2017, 32(2): 125-139.

DOI：10.1590/s0102-865020170205

• Achilles Tendon
• Amnion
• Inflammation
• Collagen
• Rats

Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon.
Methods: Sixty rats were divided into 3 groups: C- surgical procedures without tendon lesion and with simulation of hAM application; I- surgical procedures, tendon injury and simulation of hAM application; T- surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy.
Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C.
Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.

• 出版日期2017-2