Abnormal myelinogenesis both in the white and gray matter of the attractin-deficient mv rat

作者：Izawa Takeshi; Yamate Jyoji; Franklin Robin J M; Kuwamura Mitsuru^*

来源：Brain Research, 2010, 1312: 145-155.

DOI：10.1016/j.brainres.2009.11.027

• Attractin
• Myelinogenesis
• Hypomyelination
• Myelin protein
• Gray matter

The myelin vacuolation (mv) rat exhibits hypomyelination and vacuole formation in the myelin throughout the CNS, caused by a null mutation in the attractin gene. Myelin alterations in the spinal cord of my rats progress during postnatal development and are more prominent in the white matter. In contrast, microglial activation is confined to the gray matter of my rats. We here investigate the distribution and expression patterns of major CNS myelin proteins in the spinal cord of my rats during the development of the myelin lesions. Immunohistochemical and Western blot analyses demonstrated a considerable reduction in the expression of major CNS myelin proteins both in the white and gray matter of my rats, which was consistent with the morphological alterations of myelin sheaths. Real-time PCR analysis revealed a significant decrease in expression of proteolipid protein (PLP) mRNA both in the white and gray matter of my rats. However, there was no significant difference between control and my rats in the cell number of PLP mRNA-positive oligodendrocytes either in the white or gray matter, suggesting an impairment of myelin protein production by oligodendrocytes. Our results indicate that myelinogenesis but not oligodendrogenesis is severely altered both in the white and gray matter of my rats.

• 出版日期2010-2-2