Endothelial Cell Migration During Murine Yolk Sac Vascular Remodeling Occurs by Means of a Rac1 and FAK Activation Pathway In Vivo

作者：Enciso Josephine M^*; Konecny Christine M; Karpen Heidi E; Hirschi Karen K

来源：Developmental Dynamics, 2010, 239(10): 2570-2583.

DOI：10.1002/dvdy.22389

• Raldh2 mutant mice
• endothelial cell
• migration
• yolk sac
• vascular remodeling
• retinoic acid
• focal adhesions
• vinculin
• FAK
• paxillin
• Rac1
• WAVE2
• RhoA

The molecular mechanism(s) controlling cell migration during vascular morphogenesis in vivo remain largely undefined. To address this within a physiological context, we used retinaldehyde dehydrogenase-2 (Raldh2) null mouse embryos and demonstrate that retinoic acid (RA) deficiency results in abnormal yolk sac vascular remodeling due to decreased Rac1 activation, increased RhoA activation, and increased focal adhesions. Vinculin was increased in Raldh2-/- yolk sacs, and molecular events important for focal adhesion turnover, FAR phosphorylation (Tyr397) and FAK-paxillin association, were decreased. RA-rescue of vascular remodeling down-regulated vinculin and restored FAK phosphorylation (Tyr397) and FAK-paxillin association. Furthermore, vascular rescue with vascular endothelial growth factor-A, Indian hedgehog, and basic fibroblast growth factor restored FAK phosphorylation (Tyr397) in the endothelium of Raldh2-/- yolk sacs. Our results provide new insights into the regulation of endothelial cell migration during vascular remodeling in vivo by adding the Rac1 and FAK activation pathway as a critical mediator of focal adhesion formation and turnover during vascular remodeling. Developmental Dynamics 239:2570-2583, 2010.

• 出版日期2010-10