High-throughput screening approaches to identify regulators of mammalian autophagy

作者：Joachim Justin; Jiang Ming; McKnight Nicole C; Howell Michael; Tooze Sharon A^*

来源：Methods, 2015, 75: 96-104.

DOI：10.1016/j.ymeth.2015.02.002

• Autophagy
• Autophagosome
• High-throughput screens
• siRNA
• Drug and compound libraries
• Amino acid starvation
• GFP-LC3
• p62
• Z-score

This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets.

• 出版日期2015-3-15