Antitumor effects of galectin-3 inhibition in human renal carcinoma cells

作者：Xu, Yangyang; Li, Changfu; Sun, Jiahang; Li, Jingshu; Gu, Xin^*; Xu, Wanhai^*

来源：Experimental Biology and Medicine, 2016, 241(13): 1365-1373.

DOI：10.1177/1535370216630839

• Galectin 3
• renal carcinoma cells
• cell cycle
• p27

Galectins are thought to be prognosticators for survival in renal cell cancer. However, the biological activity of galectin-3 (Gal-3) in renal carcinoma cells is still debated. In this study, immunohistochemical staining confirmed a high expression of Gal-3 in tumor tissue from renal cell carcinoma. Critically, Gal-3 expression was related to tumor cell differentiation. Consistent with Gal-3 expression in renal cell cancer, strong expression of Gal-3 was also observed in several renal tumor cell lines but not in normal renal cells. A Gal-3 high-expression cell line Caki-1 was chosen to study the biological activity of Gal-3. Using short hairpin RNA method, Gal-3 expression in Caki-1 cells was knocked down. We evidenced that Gal-3 knockdown inhibited cell proliferation and invasion, induced Caspase-3-dependent apoptosis and arrested cell cycle at G1 phase. Mechanically, Cyclin D1 expression decreased, but p27 increased after Gal-3 knockdown. Taken together, these results suggest that Gal-3 is related to the development of renal cell cancer and could serve as a target to therapy renal cell cancer.

• 出版日期2016-7
• 单位哈尔滨医科大学