Allergenicity potential of red kidney bean (Phaseolus vulgaris L.) proteins in orally treated BALB/c mice and passively sensitized RBL-2H3 cells

作者：Kumar Sandeep; Sharma Akanksha; Verma Alok K; Chaudhari B P; Das Mukul; Jain S K; Dwivedi Premendra D^*

来源：Cellular Immunology, 2013, 284(1-2): 37-44.

DOI：10.1016/j.cellimm.2013.07.001

• Legumes
• Allergy
• Transcription factors
• Allergic mediators
• Thymic stromal lymphopoietin

Red kidney bean (Phaseolus vulgaris L) is one the most commonly consumed legumes that requires an in depth understanding of its allergenicity. Therefore, the aim of this study was to explore the allergenicity of red kidney bean proteins following oral exposure in BALB/c mice and elucidate the levels of Th1/Th2 transcription factors induced by red kidney bean proteins in rat basophilic leukemia cells (RBL-2H3 cells) passively sensitized with the sera of red kidney bean sensitized mice. Red kidney bean proteins showed enhanced levels of total and specific IgE, anaphylactic symptoms, thymic stromal lymphopoietin (TSLP) and peritoneal albumin over control. Enhanced release of beta-hexosaminidase along with up regulated expressions of GATA-3, STAT-6, T-bet, c-MAF and NFAT were observed in the RBL-2H3 cells exposed with red kidney bean proteins when compared to that of the controls. Taken together, exposure of red kidney bean proteins may cause allergic symptoms in mice and the ambivalent effect on Th2/Th1 transcription factors in RBL-2H3 cells.

• 出版日期2013-8