Designability landscape reveals sequence features that define axial helix rotation in four-helical homo-oligomeric antiparallel coiled-coil structures

作者：Szczepaniak Krzysztof; Lach Grzegorz; Bujnicki Janusz M; Dunin Horkawicz Stanislaw^*

来源：Journal of Structural Biology, 2014, 188(2): 123-133.

DOI：10.1016/j.jsb.2014.09.007

• Coiled-coil domains
• Protein design
• Protein modeling
• Bioinformatics
• Sequence-structure relationship

Coiled coils are widespread protein domains comprising a-helices wound around each other in a regular fashion. Owing to their regularity, coiled-coil structures can be fully described by parametric equations. This in turn makes them an excellent model for studying sequence-structure relationships in proteins. Here, we used computational design to identify sequence features that determine the degree of helix axial rotation in four-helical homo-oligomeric antiparallel coiled coils. We designed 135,000 artificial sequences for a repertoire of backbone models representing all theoretically possible axial rotation states. Analysis of the designed sequences revealed features that precisely define the rotation of the helices. Based on these features we implemented a bioinformatic tool, which given a coiled-coil sequence, predicts the rotation of the helices in its structure. Moreover, we showed that another structural parameter, helix axial shift, is coupled to helix axial rotation and that dependence between these two parameters narrows the number of possible axial rotation states.

• 出版日期2014-11