摘要
Sex-linked genetic markers of Silene latifolia were mapped to the sex chromosomes with the aim of analyzing their evolutionary origins. SIAP3X/Y has been particularly uncertain for years. Its X-counterpart has not been identified, and very few Y-deletion mutants delete SIAP3Y. In this study, we verified the sex chromosome-linkage of SIAP3X and SIAP3Y through cloning, sequencing, and fluorescent in situ hybridization (FISH). SIAP3X, spanning a region of 1.6 kb, consists of 6 exons and 5 introns. SIAP3Y has 7 exons and 6 introns, the second intron being particularly large (24.4 kb). S1AP3Y spans 31 kb in total. Their Harr-plot analysis and the chromosome positions indicated by FISH using their own probes support the hypothesis that the translocation of SIAP3X/Y has occurred on either chromosome.
- 出版日期2010-9