Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests

作者：Burgess B A; Weller C B; Pabilonia K L; Bolte D S; Van Metre D C; Morley P S^*

来源：JOURNAL OF VETERINARY INTERNAL MEDICINE, 2014, 28(6): 1853-1859.

DOI：10.1111/jvim.12440

• Culture
• Horse
• Lateral flow immunoassay

BackgroundSalmonella enterica can significantly impact management of animal facilities. Comprehensive screening is essential for effective control in high-risk populations. Availability of reliable point-of-care diagnostic tests would facilitate these efforts. %26lt;br%26gt;Hypothesis/ObjectivesCompare the ability of commercially available rapid diagnostic assays (2 lateral flow immunoassays [LFIs], DNA hybridization [DNAH], real-time PCR [qPCR]), and culture to detect common serotypes of S.enterica in feces. %26lt;br%26gt;Animalsn/a. %26lt;br%26gt;MethodsIn an experimental study, 112 S.enterica isolates were randomly selected from the 10 most common serotypes recovered at a veterinary hospital. Archived isolates were amplified in broth and standardized inocula (100 colony forming units) were incubated with equine feces in tetrathionate broth (TET). Cultures were tested in a blinded fashion by using LFIs, DNAH, qPCR, and culture. %26lt;br%26gt;ResultsThe LFIs detected 84% and 67% of isolates, respectively, but reactivity varied among serotypes. Both reacted poorly with serotype Cerro (Group K) isolates, and 1 LFI did not react with any serotype Mbandaka (Group C1) or Montevideo (Group C1) isolates. DNAH detected 94% of isolates, whereas culture and qPCR most reliably detected all serotypes. False-positive results were obtained for 4 negative controls by using DNAH and 1 negative control by using qPCR, but LFIs and culture had no false-positive results. %26lt;br%26gt;Conclusions and Clinical ImportanceCulture, qPCR, and DNAH were effective in detecting most Salmonella isolates, but have limited application at point-of-care settings. LFIs are appealing as point-of-care tests because of low cost and ease of use, but limited detection of some serotypes needs to be evaluated with samples obtained from naturally infected animals.

• 出版日期2014-12