Direct Determination of Danofloxacin and Flumequine in Milk by Use of Fluorescence Spectrometry in Combination with Partial Least-Squares Calibration

作者：Murillo Pulgarin J A; Alanon Molina A; Boras N

来源：Journal of Agricultural and Food Chemistry, 2013, 61(11): 2655-2660.

DOI：10.1021/jf3046285

• danofloxacin
• flumequine
• milk
• partial least-squares multivariate calibration
• fluorimetry

A new method for the simultaneous determination of danofloxacin and flumequine in milk samples was developed by using the nonlinear variable angle synchronous fluorescence technique to acquire data and a partial least squares chemometric algorithm to process them. A calibration set of standard samples was designed by combination of a factorial design with two levels per factor and a central star design. Whey was used as the third component of the calibration matrix. In order to assess the goodness of the proposed method, a prediction set of 11 synthetic samples was analyzed, obtaining recovery percentages between 96.1% and 104.0%. Limits of detection, calculated by means of a new criterion, were 0.90 and 12.4 ng mL(-1) for danofloxacin and flumequine, respectively. Finally, the simultaneous determination of both fluoroquinoles in milk samples containing the analytes was successfully carried out, obtaining an average recovery percentage of 99.3 +/- 4.4 for danofloxacin and 1007 +/- 4.4.

• 出版日期2013-3-20